@article{90486,
  keywords = {Bacterial Proteins, signal transduction, Quorum Sensing, Vibrio, 4-Butyrolactone, Gene Expression Regulation, Bacterial, Lactones, Microscopy, Fluorescence, Homoserine, Microscopy, Phase-Contrast},
  author = {Tao Long and Kimberly Tu and Yufang Wang and Pankaj Mehta and Ong and Bonnie Bassler and Ned Wingreen},
  title = {Quantifying the integration of quorum-sensing signals with single-cell resolution.},
  abstract = { Cell-to-cell communication in bacteria is a process known as quorum sensing that relies on the production, detection, and response to the extracellular accumulation of signaling molecules called autoinducers. Often, bacteria use multiple autoinducers to obtain information about the vicinal cell density. However, how cells integrate and interpret the information contained within multiple autoinducers remains a mystery. Using single-cell fluorescence microscopy, we quantified the signaling responses to and analyzed the integration of multiple autoinducers by the model quorum-sensing bacterium Vibrio harveyi. Our results revealed that signals from two distinct autoinducers, AI-1 and AI-2, are combined strictly additively in a shared phosphorelay pathway, with each autoinducer contributing nearly equally to the total response. We found a coherent response across the population with little cell-to-cell variation, indicating that the entire population of cells can reliably distinguish several distinct conditions of external autoinducer concentration. We speculate that the use of multiple autoinducers allows a growing population of cells to synchronize gene expression during a series of distinct developmental stages. },
  year = {2009},
  journal = {PLoS Biol},
  volume = {7},
  pages = {e68},
  month = {03/2009},
  issn = {1545-7885},
  doi = {10.1371/journal.pbio.1000068},
  language = {eng},
}