@article{200816,
  keywords = {Cryoelectron Microscopy, Organelle Biogenesis, Algal Proteins/metabolism, Carbon Dioxide/metabolism, Chlamydomonas reinhardtii/chemistry/*cytology/metabolism, Chloroplasts/chemistry/metabolism/*ultrastructure, Ribulose-Bisphosphate Carboxylase/metabolism, CO(2) concentrating mechanism, Chlamydomonas reinhardtii, Rubisco, biological phase transitions, carbon fixation, cryo-electron tomography, liquid-like organelles, magic numbers, organelle inheritance, pyrenoid},
  author = {E. S. Freeman Rosenzweig and B. Xu and L. Kuhn Cuellar and A. Martinez-Sanchez and M. Schaffer and M. Strauss and H. N. Cartwright and P. Ronceray and J. M. Plitzko and F. F{\"o}rster and N. S. Wingreen and B. D. Engel and L. C. M. Mackinder and M. C. Jonikas},
  title = {The Eukaryotic CO(2)-Concentrating Organelle Is Liquid-like and Exhibits Dynamic Reorganization},
  abstract = { Approximately 30\%-40\% of global CO(2) fixation occurs inside a non-membrane-bound organelle called the pyrenoid, which is found within the chloroplasts of most eukaryotic algae. The pyrenoid matrix is densely packed with the CO(2)-fixing enzyme Rubisco and is thought to be a crystalline or amorphous solid.~Here, we show that the pyrenoid matrix of the unicellular alga Chlamydomonas reinhardtii is not crystalline but behaves as a liquid that dissolves and condenses during cell division. Furthermore, we show that new pyrenoids are formed both by fission and de novo assembly. Our modeling predicts the existence of a "magic number" effect associated with special, highly stable heterocomplexes that influences phase separation in liquid-like organelles. This view of the pyrenoid matrix as a phase-separated compartment provides a paradigm for understanding its structure, biogenesis, and regulation. More broadly, our findings expand our understanding of the principles that govern the architecture and inheritance of liquid-like organelles. },
  year = {2017},
  journal = {Cell},
  volume = {171},
  number = {1},
  pages = {148-162.e19},
  month = {09/2017},
  isbn = {0092-8674 (Print)0092-8674},
  language = {eng},
}